WebWe have developed a Robust Activity Marking (RAM) system that allows for the identificatio... Cite Download full-text Npas4 Regulates a Transcriptional Program in CA3 Required for Contextual... WebWe have developed a Robust Activity Marking (RAM) system that allows for the identification and interrogation of ensembles of neurons. The RAM system provides …
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WebWe have developed a Robust Activity Marking (RAM) system that allows for the identificatio... Cite Download full-text Contexts in source publication Context 1 ... an optimized titer, more than... WebJul 18, 2024 · RAM: robust activity marking, d2tTA: tetracycline-controlled transactivator fused to a degradation domain, TRE: tTA-responsive element, Dox: doxycycline. We then prepared AAVs encoding the Fos-responsive, RAM-controlled Gluc-RMA and delivered them into the left CP of mice, along with Cluc-RMA and hM3Dq (Supplementary Fig. 6a).
WebA robust activity marking system for exploring active neuronal ensembles. Sorensen AT, Cooper YA, Baratta MV, Weng FJ, Zhang Y, Ramamoorthi K, Fropf R, LaVerriere E, Xue J, … WebMar 17, 2024 · The widely used activity marker F os mediates long-term potentiation of excitatory synapses ( Fleischmann et al., 2003 ). Npas4, a neuron-specific IEG that is selectively activated by neuronal activity, differs from Fos in that it preferentially recruits inhibitory synapses, or sometimes diminishes excitatory synapses, onto excitatory neurons (
WebJun 6, 2024 · Robust is a characteristic describing a model's, test's or system's ability to effectively perform while its variables or assumptions are altered, so a robust concept can … Webdspace.mit.edu
WebOct 23, 2024 · Robust activity marking (RAM) system is another method, such as E-SARE, that exploits a synthetic promoter to investigate activity-dependent cells. This strategy has been used to label and manipulate neuronal ensembles in the hippocampus of animals subjected to contextual fear conditions (CFC) and in the amygdala following tone-fear ...
WebA robust activity marking system for exploring active neuronal ensembles. Sorensen AT, Cooper YA, Baratta MV, Weng FJ, Zhang Y, Ramamoorthi K, Fropf R, LaVerriere E, Xue J, Young A, Schneider C, Gotzsche CR, Hemberg M, Yin JC, Maier SF, Lin Y Elife. 2016 Sep 23;5. pii: e13918. doi: 10.7554/eLife.13918. PubMed Article Plasmids from Article brian jesus zarateWebSep 23, 2016 · We have developed a Robust Activity Marking (RAM) system that allows for the identification and interrogation of ensembles of neurons. The RAM system provides unprecedented high sensitivity and selectivity through the use of an optimized synthetic … National Center for Biotechnology Information brian jepsonWebMar 7, 2024 · Here we report that the activity-dependent transcription factor Npas4 selectively regulates the structure and strength of MF-CA3 synapses by restricting the number of their functional synaptic contacts without affecting the other synaptic inputs onto CA3 pyramidal neurons. brian jeremy gonzalez jet of bloodWebFigure 6. The RAM system in rats and flies. (a–d) RAM labels active neuronal ensembles in rats exposed to inescapable stress (IS). (a) Schematic timeline of the experimental … brian jesus calzadaWebMar 1, 2024 · The methods most commonly used to investigate neuronal activity are based on (1) activity-driven expression of immediate early genes (IEGs), (2) transient increases in intracellular Ca2+ concentration, and (3) synaptic events such as vesicle release. brian jezephWebSep 23, 2016 · Known as Robust Activity Marking (RAM), the new system consists of a specially designed DNA sequence that is switched on by neuronal activity. Compared with … brian jesselWebSep 23, 2016 · A Robust Activity Marking (RAM) system that allows for the identification and interrogation of ensembles of neurons, providing unprecedented high sensitivity and … brian jewell nj