Hoechst excitation

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August undefined, 2024

WebFluorescence SpectraViewer DAPI (4′,6-diamidino-2-phenylindole) is a blue-fluorescent DNA stain that exhibits ~20-fold enhancement of fluorescence upon binding to AT regions of dsDNA. It is excited by the violet (405 nm) laser line and is commonly used as a nuclear counterstain in fluorescence microscopy, flow cytometry, and chromosome staining. WebEpi Red LED Excitation . Turns “ON” or “OFF” the 640 nm overhead Red lighting (for excitation of Cy5, Cy5.5 and Alexa 647) 8 . Epi UV Excitation . Turns “ON” or “OFF” the 365 nm overhead UV lighting (for excitation of QDot, wtGFP, Hoechst Blue and …

Side population analysis using a violet-excited cell-permeable DNA …

WebJan 3, 2024 · The solid white circle represents the average value of the box, and the line connects the average value of the box, respectively. Excitation wavelength and emission filters: NHCouI: E x / E m 405/500–550 nm; E x / E m 561/570–620 nm. ThT: E x / E m 488/500–550 nm. Hoechst 33342: E x / E m 405/420–470 nm. Data represent means ± … WebHoechst dyes 33258 and 33342 have similar excitation and emission spectra and are also used to stain nuclei and chromosomes. It has been reported that exposure to UV can … skelwith bridge hotel

Hoe 33342 - an overview ScienceDirect Topics

WebHoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed … Web2 days ago · Stained cells were visualized using a Nikon A1R Confocal Microscope (SickKids Imaging Facility, Toronto) with excitation wavelengths of 405 and 568 to detect signals from Hoechst 33342 and CSRBnps respectively. The laser power of CSRBnps excitation wavelength was kept constant for image acquisition of all samples. WebDNA complex excitation maximum = 355 nm DNA complex emission maximum = 465 nm Product Description Hoechst 33342 (bisBenzimide H33342 trihydrochloride) is supplied as a lyophilized powder in 25 mg … svg weather icons

Fluorescence Filter Types Chroma Technology Corp

Spectrum [Hoechst 33342] AAT Bioquest

WebApr 18, 2024 · Hoechst is widely used in co-staining applications that simultaneously visualize DNA and other cellular structures or specific proteins. Its excitation/emission spectra do not overlap with other commonly used small-molecule fluorophores and fluorescent proteins that emit in the green-red range. WebOct 25, 2024 · Hoechst 33258 staining was used to observe the effect of E. officinalis on NHDF cell apoptosis 72 h after exposure to 144 mJ/cm 2 of UVB ... These cells were placed away from light for 30 min at room temperature and then inspected using flow cytometry with excitation at 490 nm and emission at 525 nm (FACSCaliburTM; Becton-Dickinson, … svg weather forecastWebExcitation Wavelength Range 647 nm Form Liquid Product Line Pierce™ Purity or Quality Grade > 99% Quantity 200 μL Solubility Soluble at working concentrations in water and typical buffers Sub Cellular Localization … skema business school - campus de lille 59

"WebThis filter set is recommended when studying various combinations of fluorophores chosen from the following three groups: DAPI, blue fluorescent protein ( BFP ), or Hoechst dyes (violet excitation); FITC, green fluorescent protein ( GFP ), Cy2, or Alexa Fluor 488 (blue excitation); TRITC, DsRed, Cy3, Alexa Fluor 546, or Mito Tracker Red CMXRos … " - Hoechst excitation

Hoechst excitation

WebHoechst 33342 is used for specifically staining the nuclei of living or fixed cells and tissues. This stain is commonly used in combination with 5-bromo-2'-deoxyuridine (BrdU) … WebExcitation emission spectrum of Hoechst dye. Both dyes are excited by ultraviolet light at about 350 nm and both emit blue-green fluorescence with a maximum emission spectrum of 461 nm. Unbound dyes have maximum fluorescence emission in the 510-540 nm range. Hoechst stains can be excited with xenon or mercury arc lamps or UV lasers.

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WebHoechst 33342 is a bis-benzimide derivative that binds to AT-rich sequences in the minor grove of double-stranded DNA. The emission is very bright, and for the purposes of the SP … Webshift excitation spectra to the red to decrease background levels caused by autoﬂuorescence, and to develop spectral pairs of GFP that display FRET (4–6). There are vast ... Hoechst 33342 ﬂuorescence was collected at 460 nm using a 475 nm dichroic long pass ﬁlter and a 460/20 nm band pass ﬁlter. Topaz (yellow)-GFP ﬂuorescence was ...

WebHoechst 33342 side population (SP) analysis is a common method for identifying stem cells in mammalian hematopoietic and nonhematopoietic tissues. Although widely employed … WebNucleic Acid Dyes Max. excitation Max. emission Excitation laser wavelength (nm) wavelength (nm) lines (nm) DAPI 359 461 325,360,405,407 Hoechst 33258 352 461 Hoechst 33342 350 461 SYTOX Blue 431 480 YOYO-1 491 509 SYTOX Green 504 523 TOTO-1, TO-PRO-1 509 533 Mithramycin 450 570 SYTOX Orange 547 570 Chromomycin A3 445 575 …

WebExcitation emission spectrum of Hoechst dye. Both dyes are excited by ultraviolet light at about 350 nm and both emit blue-green fluorescence with a maximum emission spectrum … WebThe wavelength of excitation is critical to the total photons of light that the fluorophore will absorb. Fluorescein isothiocyanate (FITC), for example, will absorb light from 400-530 nm but absorbs most efficiently at its peak or excitation maximum of 490 nm wavelength.

WebInvitrogen Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for cell cycle studies in combination with …

skema business school chinaWebFeatures of Hoechst 33342 Fluorescent Stain: • Hoechst dye—blue fluorescent stain specific for DNA (i.e., nuclei of eukaryotic cells) • Convenient—provided as an easy-to-use Hoechst … skema business school astWebHoechst 33342 is a bis-benzimide derivative that binds to AT-rich sequences in the minor grove of double-stranded DNA. The emission is very bright, and for the purposes of the SP strategy, is detected at two emission wavelengths after UV excitation: Hoechst Red (630–650 nM) and Hoechst Blue (405–450 nM). skelwith caravan park amblesideWebThe Hoechst stains are a family of fluorescent stains for labeling DNA in fluorescence microscopy. Because these fluorescent stains label DNA, they are also commonly used to … skelwith cottagesWebThis filter set is designed for optimal detection of DAPI, FITC (fluorescein isothiocyanate), and TRITC (tetramethylrhodamine isothiocyanate) probes when applied in combination, … svg wedding gift cardWebHoechst 33342 is a well-characterized blue-emitting fluorescent compound widely utilized for nuclear staining. Hoechst 33342 permeates cell membranes and binds to the minor … svg wedding cardWebThe Nikon dual excitation band DAPI-FITC filter set is designed for the simultaneous detection of the popular fluorophores DAPI and FITC (fluorescein isothiocyanate) when applied in combination, and … skema box mini scoop 15 inch