WebMolecular Devices FLIPR Tetra User Manual (302 pages) High Throughput Cellular Screening System Brand: Molecular Devices Category: Laboratory Equipment Size: 9.46 MB Table of Contents Table of Contents 3 Chapter 1: System Overview 13 Fluorescence Mode 13 Luminescence Mode 14 System Requirements 14 Electrical 14 Minimum … WebMay 7, 2013 · Past studies indicate that CFTR activation results in altered membrane potential, which can be detected using an appropriate probe. 10 In this report, a rapid FlexStation-based fluorescent membrane potential (FMP) assay to monitor CFTR function in a microplate format is described.
FLIPR Tetra Device Driver User Guide - Agilent Technologies
WebThe FLIPR Membrane Potential Assay Kit includes homogenous florescence-based formulations for observation of real time membrane potential changes.Cellular assays … WebFLIPR Assay Cells loaded with membrane potential dye were preincubated for 5 minutes with Loreclozole, Tracazolate, MefenamicAcid and Diazepam, then stimulated with an EC 10concentration of GABA, or a dilution series of GABA. All of the PAMs preferentially activated GABA A β2* over β1*. GABA Conc (uM) 0.1 10 % n simplified stories
Phenotypic profiling of CFTR modulators in patient …
WebAbcam's Cellular Membrane Potential Assay Kit (Fluorometric - Orange) (ab176764) is a homogeneous assay with fast read time. It uses a proprietary long wavelength membrane potential indicator to detect the membrane potential change that is caused by the opening and closing of the ion channels. WebMeasuring changes in membrane potential of ion channel targets. ... Brimacombe K, et al., editors. Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the … WebMembrane Potential Indicators. Increases and decreases in membrane potential play a central role in many physiological processes, including nerve-impulse propagation, muscle contraction, and cell signaling. … simplified storagemod