Web3T3L1 adipocytes were plated in a 96-well plate at 50,000 cells/well/100 µl DMEM/FBS for 5 hours, and then deprived of serum for 1 hour with or without leptin (100 nM). Then add 10 nM insulin to the wells and incubate for another 30 minutes in a 37°C, 5% CO2 incubator. WebMade from high-quality USP Class VI virgin polystyrene, solid Nunc 96-well plates (in white and black) are ideal for colorimetric, fluorescence-, and luminescence-based cell analysis as well as protein binding assays Jump to: Clear, flat bottom, 96-well non-treated plates Black, flat bottom, polystyrene 96-well non-treated plates
96 DeepWell plate, 2 mL/well, sterile, natural, 60/cs - Sigma-Aldrich
Web96 well plates are made from virgin polystyrene. German cover glass or glass-like cover slip of superior optical quality A USP class VI adhesive is used to assemble the cover glass and the plate. Individually packed in easy to open trays Sterilized by Gamma radiation. Conforms to ANSI/SBS 1-2004 standards Cited publications before 2024 (52) WebVWR® 96-Well Deep Well Plates. Supplier: VWR, part of Avantor. Heavy polypropylene deep well plates feature square wells, printed alphanumeric labeling, and smooth interior … scotch brite tube
Does anybody have measure fluorescence in a plate reader?
Web96-Well plates are available in various materials such as aluminum and topas with glass inserts, as well as polypropylene plates, ranging in sizes to accommodate specific … WebMay 5, 2024 · Using the FlexDrop, dispense 1,200 μL – minus the volume needed for 9,600 cells – into twelve 96-well deep well plates. Automated Cell Lifting and Splitting procedure for one 96-well plate of transfected cells: Lift the cells (Trypsinize) each plate of transfected cells using the Janus 96-Tip MDT with a fresh box of P235 tips loaded. WebTwo cell lines are co-cultured in a 96-well plate, cell line A is eGFP-tagged and B is not. To see whether A line is affected by B (after specific treatments), I need to check eGFP signals in a... prefix artinya