Bioassay of cytokinin

WebThe activity, uptake and metabolism of cytokinin metabolites was determined in soybean (Glycine max (L.) Merr.) callus tissue. The following activity sequence was established: zeatin riboside (ZR)>zeatin (Z)>O-glucosides of Z, ZR and their dihydro derivatives>lupinic acid (an alanine conjugate of Z)>7- and 9-glucosides of Z which were almost inactive. WebXanthium expresses a large and rapid cytokinin response which can be obtained in very small solution volumes, and which is sensitive to cytokinin concen-trations as low as 10 …

Methodology for the Extraction, Purification, and Determination of ...

WebNational Center for Biotechnology Information WebMay 1, 1972 · The cytokinin bioassay based upon these two effects is simple and provides results after a relatively short time. The root length is measured after 6 days of germination, while a-amylase activity is determined one day later in the same barley seedlings. The parallelism of these two effects indicates, better than a single test does, the presence ... lithium redarc https://southcityprep.org

Phenylurea cytokinins assayed for induction of shoot …

WebThe name cytokinin is because of its specific effects on cytokinesis. Kinetin and certain other substances show kinetin-like activity, collectively called cytokinins. ... and a three times higher CK content as judged from a bioassay of root exudate. Since cytokinins promote cell division, it is interesting that they should show binding affinity ... WebJun 21, 2024 · 3. CYTOKININ • Cytokinins are a class of purine-type phytohormones that promote – cell division, – shoot and root morphogenesis, – chloroplast maturation, – cell … WebHence, chlorophyll preservation test is a bioassay for cytokinin. Cytokinin promotes cell enlargement in the leaf tissues of certain plants. So, radish and bean leaf test have been used as bioassay. Promotion of lettuce seed germination forms the basis for another bioassay for cytokinins. Dwarf maize test is a bioassay for gibberellins as they ... lithium recycling company stock

Cotyledon Expansion as a Bioassay for Cytokinins - JSTOR

Category:Which of the following is not a bioassay for cytokinins? - Tardigrade

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Bioassay of cytokinin

(PDF) Analytical methods for cytokinins - ResearchGate

WebThe induction of shoot buds from the filamentous protonema of moss is a classic bioassay for cytokinin. While a large literature documents this response in many species of moss and for a wide range of natural and synthetic cytokinins, to date only substituted adenine cytokinins have been examined in detail. This paper shows that at least some ... WebHence, chlorophyll preservation test is a bioassay for cytokinin. Cytokinin promotes cell enlargement in the leaf tissues of certain plants. So, radish and bean leaf test have been …

Bioassay of cytokinin

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WebThe effect of 28 natural and synthetic cytokinins, including cytokinin nucleotides, the growth of soybean cotyledonary callus was investigated. Generally the nucleosides and … Webkinin bioassay. Cytokinin-like activity was determined by the radish cotyledon assay. The radish cotyledon bioassay. Radish seeds, Raphanus sativus L. cv. Okura Daikon, were germinated in darkness at 27°C on wet filter paper in plastic boxes. After about 3 days, larger cotyledons were excised from each

WebNov 1, 2015 · Abstract. The cytokinins have been implicated in many facets of plant growth and development including cell division and differentiation, shoot and root growth, apical dominance, senescence, fruit and seed development, and the response to biotic and abiotic stressors. Cytokinin levels are regulated by a balance between biosynthesis [isopentenyl ... WebAbstract A rapid bioassay for cytokinins is described. This is based on the ability of these compounds to promote markedly the expansion of radish cotyledons excised soon after seed germination. ... naturally occurring substances without cytokinin activity, induce small growth increments in the assay. Cytokinin structure-activity relationships ...

WebModifying the cytokinin content in plants is a means of improving plant productivity. Here, we report the development and biological activity of compound TD-K (1-(furan-2-ylmethyl)-3-(1,2,3-thiadiazol-5-yl)urea)which is related to thidiazuron. TD-K—which exhibited extremely high antisenescence activity in the wheat leaf bioassay—and INCYDE (2-chloro-6-(3 … WebFeb 1, 1998 · The purpose of these protocols is to provide practical stepwise methods for the bioassay of cytokines using cytokine responsive cell lines. They include tables of the …

WebA rapid bioassay for cytokinins is described. This is based on the ability of these compounds to promote markedly the expansion of radish cotyledons excised soon after … lithium refiners asxWebyet rapid, bioassay for cytokinins (7). The sensitivity and specificity of several cytokinin bioassays, including those based on leaf senescence and callus formation, have been … imsa wisconsinWebCytokinin travels from the roots into the shoots thereby signaling bud growth in length. Cytokinin has shown the slow aging of plant organs by preventing breakdown of … imsa work zone certificationWebJul 23, 2024 · Cytokinin bioassay further demonstrated that S-4893 antagonized cytokinin-induced stimulation of callus formation and inhibition of root elongation. S-4893 also promoted seminal, crown and lateral ... lithium recycling company in nevadaWebA bioassay is described which is dependent upon the fact that gibberellin induced α-amylase release from barley half-seeds is proportional to the logarithm of gibberellin concentration applied. This bioassay has been successfully applied to the estimation of gibberellin-like substances in plant extracts. The bioassay has the following advantages: … ims awwaWebThe cucumber cotyledon greening bioassay is frequently used for detecting cytokinins. Beneficial modifications of the original technique included using 5-day-old … lithium recycling rateWebJan 1, 1989 · The response of the Biosynthesis of cytokinins by A. chroococcum 969 sample observed in the bioassay was compared to the response observed by a known concentration of r-zeatin. The amount of cytokinin-like compounds present was expressed in terms of the concentration of zeatin equivalents (ZE). imsa work zone traffic control